Cloned (Comment) | Organism |
---|---|
expression of the N-terminally StrepII-tagged enzyme in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H446S | site-directed mutagenesis, the mutant shows only slight dissociation and slightly reduced activity in forward and reverse reactionsactivity | Homo sapiens |
H497A | site-directed mutagenesis, the mutant shows dissociation of subunits, and highly reduced activity in forward and reverse reactions | Homo sapiens |
H497A | site-directed mutagenesis, the mutant shows only slight dissociation and retains full activity in the reverse eaction, but shows reduced reaction in the forward reaction | Homo sapiens |
I466T | site-directed mutagenesis, the mutant shows dissociation of subunits, a tetramer appears in addition to di- and monomers, and highly reduced activity in forward and reverse reactions | Homo sapiens |
I468K | site-directed mutagenesis, the mutant shows dissociation of subunits, a tetramer appears in addition to di- and monomers, the mutant shows reduced activity in the reverse reaction | Homo sapiens |
I487D | site-directed mutagenesis, the mutant shows dissociation of subunits, and highly reduced activity in forward and reverse reactions | Homo sapiens |
L492E | site-directed mutagenesis, the mutant shows only slight dissociation and retains activity | Homo sapiens |
additional information | truncation mutant DELTA 490-497 is almost inactive due to dissociation into di- and monomers | Homo sapiens |
N391P/L492E | site-directed mutagenesis, inactive mutant showing dissociation into di- and monomers | Homo sapiens |
N491P | site-directed mutagenesis, the mutant shows reduced activity in the reverse reaction | Homo sapiens |
T448K | site-directed mutagenesis, the mutant shows dissociation of subunits, a tetramer appears in addition to di- and monomers, and highly reduced activity in forward and reverse reactions | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
423700 | - |
gel filtration, recombinant enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | Homo sapiens | - |
diphosphate + UDP-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally StrepII-tagged enzyme from Escherichia coli BL21(DE3) by affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Homo sapiens | diphosphate + UDP-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | structure-function relationships analysis, overview | Homo sapiens |
octamer | octamers are formed by contacts between highly conserved amino acids in the C-terminal beta-helix | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose pyrophosphorylase | - |
Homo sapiens |
UGP | - |
Homo sapiens |
uridine diphosphate-glucose pyrophosphorylase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | a feature that discriminates UGPs of different species is the quaternary organization. While UGPs in protists are monomers, di- and tetrameric forms exist in bacteria, and the enzyme from yeast and human are octameric UGPs | Homo sapiens |
additional information | hUGPs are active in the octameric state and do not dissociate during the enzymatic cycle, structure-function relationships analysis, overview | Homo sapiens |